Antibacterial activity of Iraqi Honey against Staphylococcus aureus isolated from a wound in vitro and vivo

Abstract

Honey is among many of the natural products that have been used to treat various ailments such as burns and wound since ancient times. So this study was aimed to achieve to the effectiveness of various constration of Iraqi honey against the growth of Staphylococcus aureus by using of well agar diffusion technique in comparison with Gentamycin. Results showed a difference in the rate of zones inhibition by using Iraqi honey against staphylococcus where he scored concentration (60 mg / mm) (28±0.57) while the Gentamycin a concentration (60 microgram / ml) on the growth of Staphylococcus aureus (27.33±0.33). (15) rabbits were used, divided into 3 groups (n= 5 / group). All animals in all groups experimentally induced skin incision (30 mm) in the flank area of the body, and infected with S. aureus suspension, and treated after infected the wound (24 hrs.) according to the experimental design. The groups were treated with diluted honey at concentration (60 mg/ml), appear complete healing in 14 days of treatment and returning of hair in the wound area, in comparison with control group and the group when treated with Gentamicin ointment that required (21) days to recovery . So the honey is good as an anti -bacterial effectiveness against bacterial infections that infect wounds