Using the smear method and Real-Time PCR technique in diagnosis of Toxoplasma gondii parasite in white male rats experimentally infected by parasite

Abstract

The present study aims to isolate Toxoplasma gondii parasite and causing the experimentallyinfection and diagnosis of this infection by using the smear method and Real-Time PCRtechnique in different organs of white male rats such as brain, liver and testes . from abortedplacentae of infected women and experimentally infection of male rats then diagnosed of thisinfection by impression smear that produced . The present study included 30 white male ratsRattus norvegicus aged ( 60-70) days averaged weight (200-250)gm . tissue cysts of parasitewere presence in all prepared smears of brain ,liver and testes organs in different size andnumerous numbers ,the percentage of infection by using Real-Time PCR was 26% in brainand 20% in liver and testes .this study was recorded the possibility of isolation ofToxoplasma gondii parasite and and diagnosis by using impression smears method and Real-Time PCR technique in different tissues of the organs of the experimental animals.