Immune and non-immune diagnosis of H. pylori In Patients with ingastric ulcer (GU) and non-ulcer dyspepsia (NUD).

Abstract

Background:The roles that T helper type 1 (Th1) specific immune responses in protection from H. pylori challenge was understood. It is expected that Th2 immune responses are required for protection against extracellular bacteria, such as H. pylori. Both invasive and non-invasive tests are used in the diagnosis of Helicobacter pylori infection. The non-invasive tests avoid endoscopy and encompass the serologic and breath tests This study aimed to show important immune and non-immune tests for Helicobacter pyloriinfection diagnosis in patients . Patients and Methods:A total of one hundred seven (107) adult patients from both genders were attending Gastro Endoscopy Unit at Ramadi Teaching Hospital to undergo selective OGD from December 2012 to May 2013. Multiple mucosal biopsy specimens were taken for rapid urease test (RUT) to detect Helicobacter pylori in tissue samples. After endoscopy, blood specimen was taken from each patient to be used for serological tests including; IgG, IgM, by ELISA. Rapid Chromatographic Immunoassay test (CAS) was used for IgG against H.pylori also . Results: Present study showed that the rate of infection in males was same as in females, and increased within age group (31-50) years old, it was found thathigher positive results of CAS, and RUT for H. pylori, especially in younger adults. Findings confirmed that a significant relationship between H. pylori rapid urease test (RUT) with IgG and IgM specific for H. pylori antigen.