Abstract

Twenty Bacillus spp bacterial isolates (amylolytic enzyme producers) were obtained from 75 isolates which isolated from 12 soil samples from different parts of holly Karbala governorate , isolates number 5 and 17 were the most efficient for alpha-amylase and glucoamylase production respectively . Optimum environmental and culture condition has been determined for the production of alpha-amylase enzyme from isolate No. 5 by using the production media Soluble starch Yeast extract Trypton medium (SYT) that contains lactose with concentrate 1% as source of carbon and mixture of yeast extract and trypton with concentration 0.2% as source of nitrogen, the media wase support by NaCl2 , CaCl2 , MgSO4 , KH2PO4 and K2HPO4) with total concentration 0.26 as source of mineral salts . The primary pH adjusted to 7 , inoculum size was 5% and incubate for 24 hours in 42c̊ and 120 rpm/min. While the optimization of glucoamylase production from Bacillus licheniformis isolate no. 17 have been determined by using production medium (SYT) that contains soluble starch with concentration 1% as source of carbon and yeast extract with concentration 0.2 % as source of nitrogen. The media was supported by ( Nacl , CaCl2 , KH2PO4 and K2HPO4) with total concentration 0.26 as source of mineral salts. The The primary pH adjusted to 6.5 , inoculum size was 5% and incubate for 24 hours in 37c̊ and 120 rpm/min.

The article was added to IASJ on 2017-05-12

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