isolate and identify endophytic fungi from Leaves Eucalyptus Camaldulensis and Their Antifungal Activity against fungal pathogens.

Abstract

Laboratory experiments were carried out in the postgraduate laboratories Biology Department – College of Education for pure Sciences /Karbala University . The Study aimed to isolate and identify endophytic fungi from Leaves Eucalyptus Camaldulensis and their antifungal activity against some plant fungal pathogens.Results showed isolation of 53 isolates endophytic fungi from 120 segments of leaves representing colonization frequency of 44.17% . Isolated fungi included different species of Penicillium which prevail over other species (3 species), and two different isolates of Aspergillus , one isolate species of Alternaria, Cladosporium, Paecilomyces and Mycelia sterilia (Hyaline).Results showed variation in colonization frequencies within all species isolated from plant leaves. Dual culture methods for testing antagonistic activity of isolated endophytes against three plant fungal pathogens Fusarium oxysporium, Fusarium graminearum and Rhizoctonia solani .The results showed different antagonistic activities with different endophytes and pathogens. antagonistic activity was evaluated as percentage of inhibition of fungal pathogen. These results give indication of the efficiency of using endophytic as fungi as a biocontrol agents in agricultural application against fungal plant pathogens.