Isolation Enterohemorrhagic Escherichia coli from gall bladder of buffaloes and determine its sensitivity for antibiotics


Enterohemorrhagic Escherichia coli (EHEC) has been associated with hemorrhagic colitis, a severe form of diarrhea, and with hemolytic uremic syndrome, This study was preformed to find EHEC in gall bladder of buffaloes , (150 gall bladder) samples were collected from slaughter house and cultured on MacConky agar then identified by culture on Cefixim Tellurite Sorbitol-MacConkey, agar then tested with several types of antibiotics and serotyping of isolates to determine EHEC, in addition to conjucation methods were preformed .Results showed that 13 isolates of E.coli were obtained and 6 strainS were grown on Cefixim Tellurite Sorbitol-MacConkey agar and positive for specific antisera of (EHEC O157:H7), and all strains were only sensitive for nitrofurane, When conjucation done all recipent cell were resistant for antibiotics: (ampicillin,streptomycin,sulfonamide,trimthoprine,tetracycline,chloramphenicol,gentamycin and amikacin).