The role of bacterical inoculation, spraying with Humus and Magnesium sulfate fertilization on leaves growth and quality of Parsley Petroselinum crispum Var. Vulgare and its oxalic acid content

Abstract

A field experiment was conducted in the Najaf province during 2014 – 2015 and 2015 – 2016 seasons in Randomized Complete Block Design (R. C. B. D) as factorial experiment with three replicate. The first factor was bacterical inoculation with two levels (inoculation and non inoculation), seeds were inoculated before sowing , and added second time as activated dose on soil surface with water irrigation after 90 days of culture. Second factor was Humus fertilizers ( super humic) in three concentrations i.e. ( 0, 3 and 6m. L-1) with two sprayers first at 3 – 4 true leaves on plant, second after 20 days from the first spraying.Third factor: Hydrated magnesium sulfate ( MgSO4.7H2O Mg 10.5%) with three levels i.e. (0, 40 and 80kg. D-1) , two doses were added, first half dose added when seed sowing and the second half was added after 60 days from the first dose. Results showed that: bacterical inoculation significantly increased the total leave number, total chlorophyll and magnesium content . Meanwhile, bacterical inoculation significantly decreased the content of oxalic acid, oxalic acid/ calcium ratio, calcium and the ratio of the calcium oxalate crystals in the leaves compared with control treatment which gave the highest values for the above characteristics.Also , spraying with Humus at concentration of 3m. L-1 had a significant effect on the number of total leaves, leaves content of total chlorophyll, while a concentration of 6m. L-1 had a significant effect on leaves content of magnesium compared with control treatment which gave the lowest values. Also, spraying Humus significant , decreased the content of oxalic acid, oxalic acid/ calcium ratio and the ratio of the calcium oxalate crystals in the leaves compared with control treatment which gave the highest values.Fertilization with magnesium sulfate in the level of 80kg. D-1 significantly increased the number of total leaves, total chlorophyll and magnesium of the leaves compared with control treatment which gave the lowest values, meanwhile fertilization with magnesium sulfate significantly decreased the content of oxalic acid, oxalic acid/ calcium ratio, calcium and the ratio of the calcium oxalate crystals in leaves compared with control treatment which gave the highest values. Also the interaction between the two and three factors gave significant effects in all studied characteristics.