Evaluation the efficiency of biocide Baiarovin in the growth inhibition of the isolation of fungus Aspergillus flavus growth and the ability to produce poison Aflatoxin B1 poison


The aim of present study was to isolate and diagnose the mycotoxic fungi from rice in order to decreases them and their toxic effect. The results of isolation and identification from rice showed the dominant of four fungal species that belong to three genus, Aspergillus, Penicillium and Rhizopus. A. flavus was the most dominant follwoed by Aspergillus niger, Penicillium sp and R. stolonifer, the frequency percentages were 43.18, 32.95, 15.9and 7.95% % respectively , while the occurrence percentage were 100 ,80,33.33and 20 % respectively. The chemical tests for Aflatoxins B1 using ammonia test showed that 23 isolates out of 38 isolates from the fungus A. flavus were able to produce about 60.52% of the Aflatoxins B1. The thin layer chromatography (TLC) result found that only 14 isolates of A. flavus out of 38 isolates. were able to produce 36.84% of Aflatoxins B1.The results showed the effect of biocide Baiarovin in the inhibition of the growth of fungus A. flavus, the inhibition percentage was increased by increasing the concentration and the highest percentage of inhibition was occurred at concentration 1 g / 100 ml (56.86%) , white it reached at concentrations 0.1 and 0.5 respectively the inhibition percentage at the to 51.76 and 54.84%. As well as the results showed the efficiency of adding biocide Baiarovin in reduction aflatoxin B1 poison produced by the A. flavus . the efficiency reduction was varied which determined using shorthand estimated Spectrophotometer device. The treatment of 1 g / 100 ml of PD Media. showed the superiority in the reduction of the poison by 81.72% compared to treatment of 0.1 0.5 g / 100 ml of PD Media which reached to 70.96 and 77.41% respectively.