Detection of Macrolides Resistance Gene in Proteus mirabilis Strains Isolated from Different Clinical Specimens by Using PCR Based on mef(A) gene


Different species of Proteus mirabilis may vary in type of infections in both the community and hospital environments. Proteus mirabilis can cause a variety illnesses including urinary tract (uncommon in normal hosts), uncomplicated cystitis or pyelonephritis, wound, and blood stream infections (BSI). This study aimed to determine the macrolides resistance gene mef(A) in P. mirabilis strains isolated from different clinical samples by using PCR.This a prospective study involving the analyses of different clinical samples (i. e. sputum, wound swabs, urine and ear swabs) were collected from 86 patients suspected of bacterial infections from Al-Sader Medical City and Al-Zahraa hospital for maternity and children were cultivated to isolate P. mirabilis. Bacterial isolates diagnosed according to Baily and Scott’s Diagnostic Microbiology then further identified by Vitek® 2- system, the sequence of the primer used for detection of mef(A) gene was amplified by using Thermal Cycler. Statistical analysis was performed by using SPSS v.20. Eighty-six patients were randomly recruited; they were (65.12%) males and (34.88%) females. P. mirabilis occupies the lowest percentage 3.5 % among other microorganisms in this study. Other bacterial genera occupied different percentages there were 33.7%, 31.4%, 22.1% and 5.8 % for Pseudomonas aeruginosa, E. coli, Proteus vulgaris and Klebsiella pneumoniae. The antimicrobial activity patterns were detected using Kirby- Bauer method. Augmentin (AUG) occupied the highest value of effectiveness (93%) against the bacterial strains in comparison with other antimicrobial agent while the lowest value was (41.9%) for Ceftazidime (CAZ) (P<000). Only one strain carry the 402 mef(A) gene among the three Proteus mirabilis strains previously reported as macrolides resistant (phenotypically).