Antibiotic Susceptibility of Bacterial Isolates from Diabetes Patients with Foot Ulcers

Abstract

The study includes 40 patients infected with diabetes mellitus foot ulcer, belong to both sexes and their ages between 41-80 years, admitted to Baquba Educational Hospital. The swabs taken from patients have been cultured in media of Blood agar and MacConkey agar. The characterizations of morphology and biochemical test for isolates shows that 58 isolates of aerobic bacteria, these were as follows: Staphylococcus aureus 37.9%, Pseudomonas aeruginosa 32.8%, E.coli 22.4% and Streptococcus pyogenes 6.9%. Monobacteral infection was observed in 65% of the patients, while polybacterial infection was 35% . The peak proportion of diabetic foot ulcer was recorded between 41-50 years in both sexes. The sensitivity of isolates were tested against 15 different antibiotics according to Kirby-Bauer disc diffusion method on Mueller Hinton agar. It was obvious that Ciprofloxacin, Imipenem and Ofloxacin were more active against S. aureus followed by Cefotaxime. Augmentin, Ciprofloxacin and Imipenem were more active against S. pyogenes followed by Ofloxacin. Pseudomonas aeruginosa resisted to most of antibiotics except Amikacin and Imipenem. The more active antibiotics against E. coli were Amikacin, Imipenem and Piperacillin/ Tazobactam followed by Gentamicin and Meropenem.