Spectrophotometric methods for the determination of mesalazine using p-dimethylaminobenzaldehyde and p-anisaldehyde


Two simple, sensitive and accurate spectrophotometric methods have been developed for the determination of mesalazine in pure form and commercial dosage form. These methods are based on the reaction of mesalazine with p-dimethylaminobenzaldehyde in method A and p-anisaldehyde in method B to form an Schiff base which is a yellow compound coloured complex species in glacial acetic acid which are absorb maximally at 442 and 370 nm respectively. Beer's law is obeyed in the concentration ranges 0.1-6.25 and 0.5-12.5µgml-1 with high apparent molar absorptivities of 4.6248×104 and 1.6539×104 for methods A and B respectively. The proposed methods are applied successfully for determination of mesalazine in commercial pharmaceutical formulation. There is no interference from the excipients.