Impact of Vinblastine Sulphate on sperm parameters and histological changes of the seminiferous tubules in mice

Abstract

Background:Vinblastine Sulphate (VBL) is administered with other anticancer drugs for treatment of metastatic testicular carcinoma.Objective:The aim of this study was to investigate the effects of different concentrations of vinblastine on the outcome of sperm parameters and histological changes of the seminiferous tubules in mice.Methods:Thirty fivehealthymaturemalemice(78-) weeks old and weight of (2325-) g were involved in the present study. They were divided into six treated groups (0.092, 0.14, 0.18, 0.23, 0.28 and 0.4 mg/kg) with control group (5 mature males mice/group) according to periods of orally administration of each week throughout 6 weeks. Males were scarifiedattheendofadministrationperiodforassessmentofsperm function parameters. The testes were obtained and prepared for histological sections. Diameter of seminiferous tubules and thickness of layers of proliferated spermatogenic cells were assessed for all groups of male mice.Results:A significantdecrease(P<0.05)wasobservedinthespermconcentrationaftertreatmentwiththedoses 4, 5 and 6 when compared with control group and highly significantdecrease(P<0.001)inthesperm viability percentage was appeared for all doses groups when compared with control group. On the other hand, highly significantdecrease(P<0.001)appearedinthespermmotilityaftertreatmentwith all doses when compared with control group.Diameter of seminiferous tubules and thickness of proliferated spermatogenic cells layers were decreased gradually throughout periods of orally vinblastine administration. Highly significantdecrease(P<0.001) in the diameter of seminiferous tubules and thickness of proliferated spermatogenic cells layers was appeared following the treatment with high doses when compared with control.Conclusion:High doses of vinblastine sulphate have negative effects on sperm parameters and histological feature testis in mice.