The Bactericidal Activity of Gamma ,Beta on Vibrio cholerae resistance to Many of antibiotics (in vitro )


This study evaluated the effect of gamma , beta irradiation on Vibrio colerae isolated. The experiment included control (without exposure to irradiation) and 5 replicated doses of each gamma and beta irradiation ,which ranged (1.218×10-4 -0.320µSv) and (63.100 -96.950µSv) respectively. The total effect of gamma and beta irradiation on Vibrio cholerae viability was abrogated at (96.915177 & 63.100 µSv) by 137Cs(1µci) and 137Cs(9µci) respectively, the percentage of killing was highly (84%) and (85%) respectively and the viable cells was fewer than control. Gamma and Beta irradiation is efficient to killing Vibrio cholerae that cause many infection to human and may be cause death. The sensitivity of antibiotics of Vibrio cholerae to ten of antibiotic was tested, the results showed that percent of resistant against V. cholerae to Erythromycin , Methicillin, Ampicillin and Tetracycline was 70%, the percent of resistant to Nalidixic acid was 50%, percent of resistant to Amikacin was 10%, while percent of resistant to Chloramphenicol, Streptomycin, Ciprofloxacin and Norfloxacin was 0%.