IN SITU HYBRIDIZATION APPROACH FOR DETECTION OF EPSTEIN –BARR VIRUS & HUMAN PAPILLOMAVIRUS TYPE 16 IN A GROUP OF IRAQI WOMEN WITH CERVICAL CARCINOMA

Abstract

Aim: In order to investigate the involvement of (EBV & HPV 16) viruses in thepathogenesis of cervical carcinoma, the presence of the genome of these viruses wereinvestigated in 100 cervical biopsies, histologically classified as squamous cellcarcinomas & adenocarcinomas of uterine cervix. Methods: detection of DNA signals forboth EBV & HPV 16 by non-radioactive In Situ Hybridization technique (ISH). Results:A different percentage of positively signals for vital infection was found between the twogroups of examined DNA; EBV & HPV 16 infection in cervical carcinoma cases were52% and 70% respectively; on the contrary in normal cervix tissues a low percentage ofviral genomes presence were detected: 44% for EBV and 25% for HPV 16. Conclusions:The present study shows there is a compelling body of evidence that cervical carcinoma isrelated to human papilloma virus infection as a Co-factor and more recently Epstein-Barr virus have been proposed as candidates ( other Co-factore) for cervical neoplasia.Our data confirm the involvement of HPV16 & EBV in cervical neoplasia and suggest apossible role of these viruses in cervical cancerogenesis