PREPARATION OF WET AND PERMANENT MOUNT STAIN FOR STAINING INTESTINAL PARASITES WITH ONE STEP PROCEDURE

Abstract

Detection of intestinal parasites via routine microscopy considered technique demanding processes. Modified Schaudinns fixative copper base (CuSo4SF) and two food color powders Amaranth (A) and Brilliant blue (B) were utilized to create a new wet and permanent stain for identification of intestinal parasites. Eight positive stool specimens for intestinal parasites were suspended in CuSo4SF. Different concentrations of food color powder (A) and (B) were prepared by dissolving each in CuSo4SF to form A-CuSo4SF and B-CuSo4SF respectively. Each solution of A-CuSo4SF was mixed at equal ratio with each of B-CuSo4SF with the addition of several concentrations of Glycerol. Wet and permanent mounts were prepared by mixing each stool suspension with each staining solution at ration 1:1 and then examined microscopically. Follow up study for two years was conducted to detect the most optimum working concentration and to determine the stability of the permanent smears and the staining solution.Food color powders showed high dissolving capacity in CuSo4SF solution at room temperature. The solution composed of 1.6 % (A-CuSo4SF), 0.4% (B-CuSo4SF) and 9% glycerol showed 100% recovery for intestinal parasites, the highest staining proficiency and used effectively as both wet and permanent mount stain. This new staining solution remained stable for two years, while the permanent smears were stable for eight months