ROLE OF MAJOR FIMBRIAE (FIM A) IN ADHESION AND BIOFILM FORMATION OF LOCAL ISOLATE PORPHYROMONAS GINGIVALIS

Abstract

Background: Many previous studies indicate the role of the fimbriae of P.gingivalis in biofilm formation to biotic and abiotic surface P.gingivalis without fimbriae cannot gain entry inside epithelial cell. P. gingivalis. Fimbria-deficient mutants have a reduced ability to invade human gingival epithelial and human oral epithelial cell line.Objetive:To study the role of fimbria (fim A) isolated from P. gingivalis, local isolates in adhesion to buccal epithelial cells.Patients and methods:Thirty eight patients with chronic periodontitis enrolled for this study. Six (15%) isolates was identified as Porphyromonasgingivalis. Major fimbriae with MW (41KDa) were isolated from P5 isolate, partial purified was done byGoulbourne& Ellen. P. gingivalis(P5) was grown anaerobically,fractions were collected and proteins were measured at 280nm, Fraction with the same profile were used (Amicon,Grace& Co.,Danvers,Mass). Protein filtration was analyzed by procedure of Laemmli.Result: Pretreatment buccal cells with partial purified fimbriae reduce the number of attached bacteria in a dose dependent manner, the highest effect was seen at the fambrial concentration 40µ (8±2)bac/cell while the lowest effect was at the concentration 10 µ(29±3)bac/cell. Anti- fimbriae reduced bacterial number attached to buccal cells and inhibit biofilm formation. Effect of fimbriae on lymphocyte and neutrophils viability was examined by trypan blue.Conclusion: Specific antibodies against fimbriae could be used as a therapeutic agent against an aggressive type of these bacteria and reduce its pathogenicity. This approach therefore, serves as a new means to fight infectious diseases.