Induction of Broad Bean Plants Resistance Against Alternaria alternata Using K2HPO4 and H2O2

Abstract

The effect of different concentrations of K2HPO4 and H2O2 was tested on the mycelial growth of Alternaria alternata the causal agent of leaf spot disease of broad bean. The results indicated that all tested concentrations of the two materials (100, 200, 300, 400) mMol gave significant inhibition with the increase of concentration. The results also showed an increase in the activity of H2O2 upon and caused complete inhibition to the fungal growth at the concentration of 300 mMol (100 and 60.7%) respectively. In greenhouse experiments H2O2 caused a higher increase in shoot fresh and dry weight, high length, chlorophyll percentage, and complete significant reduction in disease severity (0.0) of the treated plant. and low activity of K2HPO4 in comparison with H2O2. The resistance of plants increased with the increase of the enzymes activity of Peroxidase, Catalase and Phenylalanine ammonia lyase. The local variety was more responsive than the French variety when treated with hydrogen peroxide substance.