Detection of Human Rhinovirus Antigen (RhV-Ag) and Total IgE in Serum Samples from Common Cold Patients

Abstract

Background: Human rhinovirus infections account for approximately 50% of common colds and up to 80% or more of all infections during respiratory epidemic outbreaks. Rhinovirus infections are considered as a major trigger of asthma exacerbations. Almost all children have experienced at least one rhinovirus infection by the age of two years. An enzyme linked immunosorbent assay (ELISA) can detect rhinovirus specific antibodies in both sera and nasal secretions of patients with a rhinovirus infection. An enzyme linked immunosorbent assay was shown to be more sensitive and reliable than the traditional neutralization test.Patients and Methods: A cross sectional study was carried out in Al- Ramadi city from the first of December, 2012 to the end of April 2013. The number of patients recruited in this study who having a common cold was 150. All of them were tested for the RhV-Ag and IgE tests. An interview was carried out with these patients using questionnaire prepared by the investigators and it's include the following: name, age, residency and occupation …etc.Results: Out of 150 patients, RhV-Ag was found in 104 (69.33%) as detected by the technique of Enzyme Linked Immunosorbent assay. The simple linear correlation coefficient between RhV-Ag and IgE readings was calculated and found to be significant (r=0.242, p<0.05). The two-sample t-test was used in order to compare means RhV-Ag and IgE between adults and children of the considered sample, the t-test revealed that means RhV-Ag and IgE of the children were significantly lower than that of the adults. Conclusion: Means of RhV-Ag and IgE were compared with respect to age groups (adults and children), in both comparisons adults found to have significantly higher means than children. IgE and RhV-Ag were found to be significantly linearly correlated.