Helicobacter pylori Specific IgE In gastric biopsies versus serum IgM and IgG Specific antibodies in dyspeptic patients

Abstract

Background: The immune response to Helicobacter pylori is a versatile group of mechanisms involving responses that are both protective and damaging to the host. H. pylori can directly bind to mast cells which produce pro-inflammatory factors which migrate and accumulate in the gastric mucosa It was found that infection with H. pylori is accompanied with increased total IgE and positive IgE specific for H. pylori in sera of some dyspeptic patients.Aims of the study: This study aimed to investigate the presence of IgE specific for H. pylori in gastric biopsies from dyspeptic patients regarding their gender. Patients and methods: Seventy six (76) adult patients from both genders were included in this study. Inclusion criteria included dyspeptic patients require upper gastrointestinal endoscopic examination. Patients were attending Gastrointestinal Endoscopy Unit at Baghdad Teaching Hospital during the period from December 2014 to May 2015. Gastric biopsies from patients with dyspepsia were investigated for IgE specific for Helicobacter pylori. using ELISA test. Sera from the patients were tested for IgM and IgG specific for H. pylori using ELISA test. Results: Study revealed that 75% of patients with serum positive IgM specific for H. pylori were showing positive IgE specific for H .pylori in their homogenates of their biopsies. While Sixteen (16) out of 60 (26.7%) positive H .pylori IgG patients were showing positive IgE specific for H .pylori in homogenates of their biopsies. Conclusion: The current study concluded that IgE specific for H. pylori undergo release locally in gastric mucosa of dyspeptic patients infected with H .pylori.