A Comparison of efficiency of (AMP) and its derivative (AMPAA) against some pathogenic bacteria


Eugenol (4-allyl-2-methoxyphenol, AMP), is a well known a biologically active phenolic componentand essential oil from Eugenia caryophyllata, which widely used of Eugenol as an antisepticand analgesic in dental care, so it is active against oral bacteria associated with dental caries andperiodontal disease as well as previous studies have shown the effect of Eugenol antifungal; anticarcinogenic;anti-allergic; anti-mutagenic activity; antioxidant and insecticidal properties, thereforeit can be used in preparation of various food as a flavouring agent and cosmetic.This study aimed to synthesize derivative new medical material 2-(4-allyl-2-methoxyphenoxy)acetic acid (AMPAA) from eugenol (4-allyl-2-methoxyphenol) (AMP) and investigate the antimicrobialactivities of both AMP and derivative component (AMPAA), The minimum inhibitoryconcentration (MIC); minimum bactericidal concentration (MBC) and sensitivity against sixpathogenic bacterial isolates: Streptococcus. pyogens; Enterococcus. faecalis; Escherichia. coli ;Klebsiella pneumoniae; Bacillus. subtilis and Proteus. mirabilis with study compare the efficiencyfor both AMP and AMPAA on the same bacterial isolate obtained from Iraqi hospitals.In this present study synthesize new medical material (AMPAA) from AMP by reacting sodiumeugenate with sodium chloroacetic acid and prepare soluble water from eugenol and its derivativeAMPAAto scanning the antimicrobial efficacy against some pathogenic bacteria isolatesby twocommon methods; well diffusion and broth dilution methods.Results of the present study show all bacterial isolates were sensitive to both AMP and AMPAAin low concentration except K. pneumoniae, also 10.0 and 5.0 μg/mL of AMP and AMPAA asMBC for bacterial isolates except K. pneumoniae and P. mirabilis that’s meaning the new derivativecompound AMPAA has more efficacy on six bacterial isolate than eugenol (AMP).By using Well diffusion method all bacterial isolates were sensitive to both (AMP and AMPAA)in low concentration, but K. pneumoniae was killed in same concentration, so all isolates havebeen killed at concentrations between (10 - 50 μg/mL) of new derivative compound (AMPAA), atthe same concentration of AMP were killed for K. pneumoniae and P. mirabilis) ,as well as allisolates have varying degrees of sensitivity towards both (AMP and AMPAA), whereas all isolateswhere more sensitive to AMPAA than AMP.In conclusion, all bacterial isolate were sensitive to both AMP and AMPAA in low concentrationexcept K. pneumoniae, also 10.0 and 5.0 μg/mL of AMP and AMPAA respectively conceder asMBC for bacterial isolate except K. pneumoniae and P. mirabilis, that killed in these concentration,so at the same concentration of AMP and AMPAA bacterial isolates were sensitivity by welldiffusion method, whilst its killed by broth method, that’s consulate broth method was best thandiffusion method.