Role of dietary antioxidant (ascorbic acid, vit.A and alfa tocopherole) in correction of liver dysfunction resulted from oxidative stress in rabbits affected experimentally with diabetes mellitus

Abstract

Determination of reliable biochemical parameters of experimentaldiabetic rabbit by alloxane monohydrates on liver function and role ofdiatery antioxidants (ascorbic acid, vit. A, and α-tocopherole)supplementation were investigated. Body weight gain, blood (plasma)chemistries, antioxidant enzymes and histopathological lesions weredetermined over 10 week in newzealand white rabbit group each contain 10animals: control G1 animals group, diabetic only G2 animals group,diabetic and ascorbic acid G3 animals group, diabetic and Vit. A G4 animalsgroup, diabetic and α-tocopherole G5 animals group . each of dietaryascorbic acid, vit. A and α-tocopherole that given to animals of G3, G4 andG5 respectively were significantly (P≤0.01) improve body weight gain andglucose level in blood than G2 diabetic group G3 and G5 significantly didnot differ but these two groups significantly more best than G4 group, alsothese antioxidants improve levels of alanine aminotransferase, aspartateaminotransferase, alkaline phosphatase and total protien. Enzymatic activityof liver glutathione peroxidase and superoxide dismutase were increasedsignificantly more than of diabetic animals G2. histopathological structuresof liver and pancrease confirm these results, as generally oxidative stressthat affect liver function may diminished by administration of antioxidantascorbic acid, Vit. A and α-tocopherole supplementation, and α-tocopherolegroup was the best group.