Abstract

Retinitis is the most important clinical consequences of Toxoplasmosis but themechanisms used by T. gondii tachyzoites to invade the retina are not yet understood. Inthe current study, cellular pathogenicity of Toxoplasmosis in a human retinal pigmentepithelial cell line (ARPE-19) was investigated. Following in vitro infection of ARPE-19with T. gondii tachyzoites, cell viability associated with infection was identified byMethyl thiazolyl tetrazolium (MTT) assay. Intracellular development of T. gondiitachyzoites within ARPE-19 was characterized by acridine orange (AO) staining.Morphological changes associated with infection was assessed by electron microscopy(EM). The results obtained showed that ARPE-19 permit the invasion, growth anddevelopment of T. gondii tachyzoites and that infection can cause a metabolic burden onthe host cells and multiple morphological changes in the relocation of the host cellorganelles (mitochondria, endoplasmic reticulum, and Golgi apparatus) around theparasitphorous vacuole

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The article was added to IASJ on 2018-05-09

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