SEOPREVALENCE AND MOLECULAR DETECTION OF BABESIA SPECIES IN CATTLE IN BAGHDAD CITY

Abstract

Cross-sectional study carried in areas surrounding Baghdad province (Dora ,Nahrawan, Tajy and Abu Graib )respectively. During June -December 2016, fordetection of cattle Babesiosis. One hundred fifty (150) clinically healthy local cattlebreeds of different ages, and from both sex were examined by routine blood smearexamination, molecular detection of Babesiosis by Conventional polymerase chainreaction PCR and by ELISA test. Results revealed that the infective species ofBabesia during this study were B.bovis and B.bigemina that had detected in 14 cases( 9.33%) by traditional blood smear examination, compared with 15 cases ( 10%)with Babesia bovis by conventional PCR technique and 100 cases (66.6%)withBabesia bigemina were detected by using ELISA technique .It has been concludedthat infection with Babesia.bovis and Babesia bigemina were more prominent and noother species were detected