The Study of Genotoxic and Cytotoxic Effects and DNA Damage by Ivermectin in Bone Marrow of Male White Swiss Mice Mus musculus


In this study the Genotoxic and Cytotoxic effects of the anti-parasites Iverrmectin (IVM) in bone-marrow of male white swiss mice Mus musculus were evaluated using the cytogenetic endpoints: Micronucleus test (MN) in Polychromatic Erythrocytes (PCEs), Chromosome aberration analysis in somatic cells and molecular cytogenetic represented by Single cell gel electrophoresis (Comet assay). The doses 0.4. 0.6 and 0.8 mg/kg.body weight . were orally administrated to the mice of treated groups, negative control group were given distelled water. The results showed that IVM induced significant decrease in the mean of polychromatic erythrocytes and the lowest value was recorded at the dose 0.6 mg/ kg. b.wt.(P≤ 0.01) and significant increase in the mean of micronuclei were induced by the three doses of drug (P≤ 0.01). IVM induced both anuegenic and clastogenic effects in bone marrow of male white mice, The results showed a significant increase in means of numerical chromosome aberrations (anueploidy and polyploidy) (P≤ 0.05), the highest mean of structural chromosome aberration (with and without gabs) was recorded in mice treated with 0.8 mg/ kg. b.wt. (P≤ 0.01) . Oral treatment of white mice with IVM induced a significant DNA damage in bone marrow cells, which revealed by comet assay results, the mean of damaged bone marrow cells in mice treated with doses 0.6 and 0.8 mg/ kg. b.wt. were different significantly when compared with negative control(P≤ 0.01). Results of current study revealed that IVM have genotoxic and cytotoxic effects on somatic cells of male white mice. the study recommend studying the genotoxicity and cytotoxcity of IVM using other animal models by other cytogenetic endpoints.