Effect of combination of crude enzyme extract of kiwifruit enriched enzyme Actinidin with green tea extract on some physico-chemical and sensory characteristics of spent layer hens meats

Abstract

This study was Designed to evaluate the efficiency of different concentrations of crude enzyme extract of kiwifruit enriched actinidin enzyme and their combination with different concentration of green tea extract enriched catechins compounds on some physico-chemical and sensory characteristics of spent layer hen meats. A total 42 spent layer hens (18 months) were used in this study. Hens were divided randomly into seven treatments (6 hens per treatment). After slaughtering and dress carcasses, breast and leg cuts were separated and then deboned removed from these cuts. Breast and leg meats were sliced into several chunks meat and weighting of 600g/treatment (6 replicates per treatment), During 30 min after slaughter, 100g of chunks meat for each replicate/ treatment were obtained from breast and leg meat were separately immerised in 100ml of cold water solutions comprised of crude enzyme extract of kiwifruit at concentrations of 5 (K1), 10 (K2) and 15 (K3) % (v/v) and their combination with green tea extract at concentration of 0.5 (T1) and 1 (T2) % (w/v), After waited for 30 min to drain for excess solutions, then pocked in polyethylene bags and stored at 4ﹾc for 24hr, then kept frozen at -18ﹾc, until the time testing. Results were shown that breast meat and leg meat samples were immerised in the crude enzyme extract of kiwifruit at concentrations of 10% with green tea extract at concentration of 1% or 15% of crude enzyme extract with 0.5% of green tea extract recorded a lower (P<0.05) cooking loss and high effective in retarding lipid oxidation (less thiobarbutric acid (TBA)) as compared with control and other treatments. Also, recorded these treatment a significant (P<0.05) in soluble collagen content and collagen solubility percentage in breast meat and leg meat samples as compared with control and other treatments. Breast meat had higher soluble collagen content, collagen solubility percentage, and lower non-soluble collagen content more that the contents of leg meat for each treatment. It was observed that breast meat and leg meat samples immerised in 10% of crude enzyme extract and its combination with 1% of green tea extract was the best in the sensory traits (flavor and aroma, tenderness, juiciness and overall acceptability) as compared with control and other treatments. It can be concluded that combination of crude enzyme extract of kiwifruit (10%) with green tea extract (1%) recorded better scores for patability traits, specialty tenderness trait and retarding of lipid oxidation of spent layer hen meats