The Antimicrobial Activity of Some Medical Plants Extracts Used Against Some Types of Bacteria that Causes Urinary Tract Infection

Abstract

This study was done to evaluated the antibacterial activity of hot ethanolic and aqueous extracts of Syzygium aromatic (S.aromatic ) and Quercus infectoria infectoria (gall) against pathogenic bacteria that causing urinary tract infection (UTI). Such as Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. Qualitative detection of active compounds was done with chemical reagents. The result showed that the extracts of Q.infectoria infectoria was composed of the saponin, tannins, flavonoids, terpenes, Resins, coumarin, glycosides, alkaloids and volatile oils. Also the Qualitative detection of the active groups was further verified with Fourier Transform Infrared Spectroscopy (FTIR). The sensitivity of bacteria was examined against 12 different antibiotics. The results show that E.coli was the most resistant bacteria, resisting 8 antibiotics; P.aeruginosa resisted 5 antibiotics; while S.aureus resistance to 4 only. The antibacterial activity of the plant extracts was investigated for each extracts against bacteria mention above, the plant extracts showed different effects on the growth of all bacterial strains. The Minimum Inhibitory Concentration (MIC) and the Minimum bactericidal concentration (MBC) of the extracts were determined. The kill- time was determined for each extract.The antioxidant activity of the plants in the study was investigated. Compiled results show that all plant extracts have antioxidant effect but that varied depending on the type of the plant and extract. The toxicity of the plant extracts was examined on human red blood cells. It was concluded that the plants do not exhibit any toxicity except for the extracts of Q.infectoria which caused the agglomeration and precipitation of the red blood cell.