FIELD EVALUATION OF BIVALENT KILLED VACCINE OF INFECTIOUS BRONCHITIS AND NEWCASTLE DISEASES PREPARED FROM LOCAL ISOLATED STRAINS OF BROILERS IN IRAQ

Abstract

This study was aimed to evaluate of oil emulsified vaccine againstinfectious bronchitis and Newcastle diseases that prepared for the first time inIraq from strains were isolated from broilers. A total (5000, 4500, 6000,4500)broiler chicks were distributed in four broilers farms in different areas of Iraq(Baghdad/Al-Taji 1, Baghdad/Al-Taji 2, Karbala, Waset) respectively whichwere previously highly infected areas with Infectious bronchitis and Newcastlediseases. The vaccinated chicks were monitored from 1 to 35 days of age. Bloodsamples were taken at (7, 14, 21, 28 and 35) days of age.Statistical results showed significant (p≤ 0.05) differences in the immuneresponse of the chicks that were vaccinated chicks with the local inactivatedbivalent vaccine (experimental vaccine) by using ELISA. The results revealed asignificant high level of antibody titer against IB and ND (each alone) in serum ofchicks that vaccinated with the experimental vaccine compared with chicks thatvaccinated with commercial inactivated bivalent vaccine at 21, 28 and 35 days ofage. These results proved the possible protection of commercial chickens byproduction oil based IB ND vaccine from locally isolated field strain.