Antibiotic resistance patterns and adhesion ability of uropathogenic Escherichia coli in children


This study aimed to isolation and characterization of E.coli from Children urine , the phenotypic detection of biofilm formation , bacteria ability to adhesion, and the detection of fimH gene using PCR-based molecular diagnostic methods. In order to isolate E.coli, 250 urine samples were collected from children in camp saad of displaced / Diyala.Fifteen isolates(30%) of Escherichia coli were recovered according to the bactereiological , biochemical tests , used Api20E system and VITEK 2 system for confirmation. The study found that bacterial growth in females higher than in males and the incidence of infection in children under the age of 3 years is more common than in older age groups. The sensitivity test was performed for Ten antibiotics (Ampicillin, Piperacillin/tazobactam, Cefoxitin, Cefixime, Ceftazidime, Imipenem, Amikacin, Gentamicin, Ciprofloxacin, Trimethoprim /sulfamethoxacole) Where the percentage of resistance were (93.3% , 20.0% , 26.7% , 80.0% , 80.0% , 0.0% , 0.0% , 46.7% , 20.0% , 73.3%) respectively.Minimal inhibitory concentrations (MIC) were determined for Ceftazidime,Piperacillin /tazobactam, Gentamicin and Trimethoprim/ sulfamethoxacole. The MIC values were (4-32), (4-128), (<16), (20-320)μg / mL.Some of the virulence factors were detected, that included detection of biofilm formation and isolates ability to adhesion, all isolates showed ability to produce a biofilm and adhesion .Gene fimH was detected by Polymerase Chain Reaction(PCR), the percentage of isolates containing the gene was 100%.