Molecular diagnosis and DNA fingerprinting based on IS6110 of Mycobacterium tuberculosis isolated from patients in Iraq

Abstract

Polymerase chain reaction assay (PCR) used for the detection of Mycobacterium tuberculosis strain and fingerprint study. Ten positive cultures on Lowenstein Jensen (LJ) media were collected from specialized center of chest and respiratory diseaseBaghdad .genomic DNA was extracted from all isolates and subjected to genetic characterization by (PCR) methods. two specific primers were used in pcr analysis for detection 16S rRNA and insertion sequence IS6110 where commonly used as a target of Mycobacterium. The result of gel electrophoresis showed that all isolates belong to Mycobacterium, and a sequence showed that 99% identity in sequence of 16S rRNA of M. tuberculosis and there is one nucleotide changes, the isolate submission in gen bank NCBI with accession number (MG03060) and 100 identity in sequence of IS6110 of M. tuberculosis. The isolate showed there is Ten polymorphism diversity according to copy number of IS6110 that have, where 80% of M.tuberculosis has large copy number (6_10) and 20% have low copy number (1-5) of isolate. This study showed that M.tuberculosis isolated in Iraq belong to two groups. Polymorphism groups depended on copy number of IS6110.