Acute Toxicity Study of Indomethacin and Oxytetracycline in Rabbits


Background: The toxic effect of nonsteroidal anti‑inflammatory drugs (NASIDs) and broad‑spectrum antibiotics in human and animal sciencehas been studied widely. The advantages and the adverse impact of these medications could be determined by measuring some biochemicalparameters such as the level of alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and bloodurea nitrogen (BUN). In addition, the changes in some hematoimmunological parameters could also be measured; for instance, red bloodcell (RBC) count, hemoglobin level, white blood cell (WBC) count, and differential WBC count. Objectives: The aim of this study is toevaluate the effect of both indomethacin, as a NSAID example, and oxytetracycline, as a broad‑spectrum antibiotic separately and together,on some biochemical and hematological parameters. Materials and Methods: Sixteen male albino rabbits were used, with age about 1 yearand body weight about 1.5 kg. They were divided into four groups and each group contains four rabbits. The first group served as a controlgroup, which receives only food and water. One of the groups were administered with oxytetracycline at a dose of 50 mg/kg. The secondwas administered with indomethacin at a dose of 5 mg/kg, and the final group was administered with both oxytetracycline and indomethacinwith the same previous dose. All the drugs were dissolved in the distilled water before administration of dose to each animal in the grouporally using a stomach cannula for 14 days. The rabbits were kept for 14 days under the room temperature with free access to food andwater. Serum was separated from the clotted blood obtained by ear vein. Total RBC and WBC counts were made by the hemocytometermethod. The differential WBC counts were made by finding the percentage average of the different types of cells counted in ten fields fromGiemsa‑stained slides. Serum enzymes such as ALP, AST, and ALT were determined by procedures of Sigma Diagnostics, BUN by method ofCrocker. Results: The level of ALP has shown great increase in all groups, while the level of AST and ALT was increased only in the groupthat administered with indomethacin. However, both parameters showed a significant decrease in the other groups. Regarding hematologicalchanges, WBC increased in all groups, while RBC and Hb level showed sharp decrease. Lymphocytopenia occurred in only the group thatadministered with oxytetracycline. Conclusion: The results showed toxic effect of these two most popular using drugs in animals specificallywhen it is used chronically. In addition, there is no great difference when it is used separate or together. Further study is required to investigatethe level of damage that happens in the organs.