Immunohistochemical expression levels of anterior pituitary LH, FSH and testicular testosterone of Allium sativum extract and thymoquinone in male treated rats


The present study was conducted to determine the immunohistochemical expression levels of Luteinizing Hormone and Follicle Stimulating Hormone in the anterior pituitary gland and testicular testosterone of male rats treated with Allium sativum extract and Thymoquinone. Forty mature Waster male rats Rattus norvegicus, aged 60 days and weighed 140-150 g, were randomly divided into control and three treatment groups (10 rats each). Control group males were orally supplemented with 500 µL of normal saline daily. The treated groups of males were orally supplemented with TQ suspension, 50 mg/ kg, B.Wt (TQ group), Allium sativum aqueous extract, 50 mg/ kg bw (A group) and their combination (TQA group). At the end of experiment, all male rats were sacrificed and pituitaries and testes were dissected and fixed in neutral formalin buffer 10% for immunohistochemical examination of pituitary LH and FSH and testicular testosterone. The results demonstrated high immunohistochemical density in treated male’s pituitary tissues for both LH and FSH compared with the control group. In comparison with control, testicular tissues of treated group males showed stronger immune reaction for T especially around Lydig’s and Sertoli cells. It can be concluded that TQ and Allium sativum have potent improving effects on reproductive hormones production and could be increase the male fertility.