The Reactivity of Anti-HCMV IgM to Various Specific HCMV Antigens Among Pregnant Women in Kirkuk.

Abstract

Human Cytomegalovirus (HCMV) is a DNA virus of the Betaherpesvirinae subfamily and Herpesviridae family. HCMV is a leading cause of congenital infections throughout the world. This study was carried out in Kirkuk governorate from July 2017 to January 2018,aimed at studying there activity of anti-HCMV IgM to various specific HCMV antigens among 180 pregnant women attending hospitals, primary health care centers and some private medical laboratories. The pregnant women were examined for HCMV-IgM seroprevalence using Electro-chemo-luminescence(ECLIA) technique then examined their reactivity for specific HCMV antigens using line immune assay. The rates of HCMV-IgM seropositive were 22 (12.22 %). Regarding the reactivity of determined HCMV-IgM against various HCMV antigens, the rates 14 (63.63%), 13(59.09%), 21(95.45%), 15(68.18%), 21(95.45%) and 16(72.72%) were seropositive for HCMV IE1, CM2, p150, p65, gB1 and gB2 antigens, respectively. Concerning the band intensity of HCMV-IgM reactions with HCMV antigens, the rates of (+++) were higher than other band intensity for all antigens with highest rate for gB1 antigen (85.71%). So, the highest rate of HCMV-IgM reaction with the number of antigens was 40.90% for three antigens at the same time. In conclusion, the highest rate of HCMV-IgM in pregnant women was for gB1and p150 antigens; while the highest rate of intensity reaction was (+++)and the highest rate of HCMV-IgM ability for reaction was for three antigens at the same time.

Keywords

HCMV, ECLIA, CM2, p150, gB.