Identification of Leishmania donovani in blood of experimentally infected rats by quantitative real-time PCR
Abstract
The present study has been carried out at the Department of Microbiology and Parasitology, College of Veterinary Medicine/Al-Qadisiyah University to diagnose the visceral leishmaniasis molecular technique (Quantitative real-time polymerase chain reaction). The experimental study includes fourth Wistar female rats (weighted 250 ± 2 g.) were injected by blood from an infected patient in the peritoneal cavity, after 8-10 days of experimental infection, blood samples had been collected directly from the heart in order to diagnose the infection by using quantitative real-time polymerase chain reaction. Quantitative Real-Time PCR qPCR technique was used for amplification of the conserved region in GAPDH gene that was used for detection of Leishmania donovani in blood samples of rats. It shows the Amplification of genomic DNA template concentrations of Leishmania donovani during reaction with syber dye inside the apparatus under threshold cycle, also shows the melting peak of Leishmania donovani genomic DNA template concentrations is demonstrate the specialization of Leishmania donovani genomic DNA amplification in a single peak for all samples.
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