Association of PPARG gene polymorphism (Pro 12 Ala) with the risk of type 2 diabetes mellitus (T2DM) incidence in sample of Iraqi patients

Abstract

Diabetes mellitus is a combination of heterogeneous disorders commonly presenting with episodes of hyperglycaemia and glucose intolerance, as a result of lack of insulin, defective insulin action, or both . PPARG gene variant ( pro 12 ala) has been linked to risk of development of diabetes mellitus . this study was carried out to examine whether the polymorphism of PPARG gene are correlated with the incidence of type 2 diabetes mellitus (T2DM) in Iraqi population. Diabetic patients (n=50) and apparently healthy control subject (n=50), were enrolled genotyping of PPARG gene SNP (rs1801282) were determined by using Taqman genotyping assay. The results showed that the distribution of genotypes and alleles frequencies at (rs1801282) SNP of PPARG gene, as related with CC , CG and combined CG+CC genotypes, G allele seem to be a protective allele, therefore, the presence of both heterozygous and homozygous mutants may reduce the risk of T2DM (the frequency of CG+GC mutants were 68% in apparently healthy control individuals and 50% in T2DM patients). In contrast, there is wild CC genotype (50 versus 32% in T2DM and control group, respectively, X2=6.93; P<0.01; OR=1.272).