Screening of Eight Potato Cultivars ( Solanum tuberosum L.) to in Vitro Growth and production of Microtuberzation Under Salt Stress Conditions Using Some Physiological parameters


The present research was conducted to study the physiological traits of potato seedling to understanding of the plant's response under salt stress conditions, which consequently serves to try to cultivate potato cultivar that are saline tolerant. Eight imported cultivars of potato seeds were selected: Almondo, Arizona, Buren, Everest, Rievira, Rudolf, Sever and Sylvana. A tissue culture was established for the branches of these cultivars to study the effect of different concentrations of sodium chloride (NaCl) on growth properties and the production of microtubers in Vitro. The concentrations (0, 50, 100, 150, 200 mmol) of sodium chloride salt were added to the media of both Murashige and Skoog (MS) each separately and two cutting were cultivated with two nodes per repeater. The results showed that Riviera cultivar showed tolerance to saline stress conditions at the salts concentrations used in terms of growth and production of micro tubers. For plant content of dry substance, Riviera cultivar gave the highest percentage of dry substance at 25.20% while the same cultivar gave the lowest percentage of 0.9% at the concentration of 200 mmol.L-1. In the control treatment, Riviera cultivar was given the highest percentage of protein 3.81%. Significant differences were observed in the effectiveness of the peroxidase enzyme. Arizona cultivar that cultivated in the control treatment (without salt) gave the least effective for enzyme 13.60 Absorption unit.g-1. Riviera cultivar that cultivated in concentration of 150 mmol.L-1 gave the highest activity for peroxidase enzyme was 38.0 absorption units.g-1.