Isolation and Identification of Candida albicans in different clinical samples

Abstract

Taghreed khudhur MohammeThe prevalence of candidacies caused by Candida albicans has increased years ago. The samples (Vaginal swabs; urethral swabs; urine; and ear swabs) were cultured on Sabourauds Dextrose Agar and CHROM agar™Candida to detect Candida albicans in patients clinically suffered from candidiasis. Six hundred and five clinical samples were collected from patients whose ages ranged from (20 – 69) years. All Candida albicans isolates were identified by using microscopic examination in wet smear and stained smear; in addition to germ tube test and API Candida. The highest rate of Candida albicans infections were 73.3% in pregnant women in age group (20 – 43) years; 43.7 % in men of (30 – 39) years old and (33.3) % in men and (37.9) % in female with urinary tract infection. Susceptibility testing of Candida albicans to six antifungal drugs was carried out by disc diffusion method; They were Amphotericin B, Nystatin, Fluconazole, Ketoconazole, Cotrimazol and Itraconazole. Resistant Candida albicans isolates resistance were found against Fluconazole (45.45) %; Ketoconazole and Itraconazole (13.6) %respectively. 100%of susceptibility was observed for Cotrimazol, Amphotericin B and Nystatin.