Study to determination minimum inhibitory concentration (MIC) values of different antibiotics against the common isolates of Enterococcus that isolated from pregnant women urine by agar dilution method.

Abstract

Bacterial invation of any part of the urinary tract is known as Urinary Tract Infection (UTI). 70 urin specimen was taken from pregnant women who had been referred to laboratories of AL- Hussein teaching hospital and Maternity teaching hospital, Karbala, between (1 December - 30 February). Isolates were identified phenotypically and species assigned according to results of biochemical tests. The results showed Enterococcus faecium was predominant, its comprising (10 %) compare with other enterococcal species, and among 5 enterococcal isolates, 2 (comprising 40%) and 1 (comprising 20%) were β and α haemolytic respectively and 2 (comprising 40%) were no haemolytic activity, gelatinase was produced by 4 isolates (comprising 80%) and 1 isolate (comprising 20%) no gelatin liquefaction. In addition to that the results showed that the number of moderate biofilm and non-biofilm producers were 2 (40%) , and weak biofilm was 1 (20%) among 5 enterococcal isolates. The MIC values of the different antibiotics (Vancomycin, Teicoplanin, Ampicillin, Erythromycin, and Ciprofloxacin) were determined against the four E . faecium isolates. The MIC values of the vancomycin against E . faecium 1, E . faecium 2, E . faecium 3, and E . faecium 4 was found to be (8 , 8 , 30.6 , and 15.3 µg/ml) respectively. The MIC values of ( teicoplanin, ampicillin, erythromycin, and ciprofloxacin) against the four E . faecium isolates was found to be (32 , 16 , 32 , and 16 µg/ml) for teicoplanin , (8 , 8 , 8 , and 15.3 µg/ml) for ampicillin , (4 , 8 , 8 , and 8 µg/ml) for erythromycin , and (2 , 1 , 1 , and 2 µg/ml) for ciprofloxacin. On the other hand, the results observed that the activity of the ciprofloxacin against the four Enterococcus faecium isolates was more effective than existing drugs. Its showed very good activity against Enterococcus faecium 2 and Enterococcus faecium 3. Teicoplanin has very low activity against the four enterococcal isolates compared with the existing drugs in our study.