Estimation of Interleukin-10 Levels as a Predictive Factor in Iraqi Non-Hodgkin’s Lymphoma Patients


Background: Interleukin-10 (IL-10) is a pleiotropic cytokine produced by type 2 helper cells (TH2), monocytes ,macrophages, and neoplastic B lymphocytes IL-10 production has a strong immunosuppressive effect, and it actsas an effective stimulator for B-cells .IL-10 level increase in Non-Hodgkin lymphoma (NHL) patients ;moreover ,this was associated with poor prognosis .Objectives: to assess the level of interleukin-10 in patients with non-Hodgkin lymphoma and to correlate its level withprognosis.Materials and Methods: The present study was carried out to evaluate IL-10 level in the sera of 46 NHL Iraqi patients,in addition to a control group involving 46 matched apparently healthy subjects using serological method theenzyme-linked immunosorbent assay (ELISA).This study was carried out at the National center of hematologyfrom January to June 2013.Results: there were 31 (67%) patients with age group ≥50 years and 15 (33%) patients with age group <50 years. Themean age of NHL patients was 52.74 years ranging from 17 to 80 years? Moreover, the study noticed a malepredominance and male to female ratio was 1.7:1.When the patients of NHL compared with control, the levels ofinterleukin 10 were markedly increased in patients with NHL (18.67 ± 4.12pg/ml vs. 3.4 ± 0.73pg/ml) (p<0.05).Higher levels of IL-10 were noticed in the advanced stage patients of compared to early stages (33.1 ± 4.5 pg. /mlvs. 13.4 ± 2.7 pg. /ml) (P<0.05).Conclusion: In the highest age of NHL patients was noticed and it might indicate the increasing rates of NHL patientsamong older population. in addition increasing level of IL10 is associated with increasing age range and alsocorrelated positively with aggressive NHL cases that might indicates the predictive pivotal role of this cytokine.