Sensitivity of treponema denticola isolated from infected periodontal pockets to some mouth rinses and common antibiotic


Aims: Treponema denticola is one of the most important periodontal pathogens because of its high lytic enzymatic activity leading to tooth lose and its ability to invade tissues and spread via blood stream causing systemic infections, this study coming to be the first local study concerning with this anaerobic fastidious bacterium and looking at the possibility of rely on the culturing methods in determining the most preferred chemotherapeutic drugs for limiting of bacterial activity and spread, as well as investigating the range of the antimicrobial activity of some mouth rinses and number of antibiotics. Materials and Methods: 139 samples were collected from periodontal pockets with a depth of 3 mm under supervision of specialist dentitist, then placed in a reduced transport medium. The activity and effective spectrum of different concentrations for three kinds of widely used mouth rinses in the treatment of periodontal infections including Biofresh K, Biofresh F and Zak towards this bacterium was studied using sensitivity test methods, furthermore, the minimum inhibitory cocentration of antibiotics amoxillin, ciprofloxacin, clindamycin, azithromycin and mitronidazole was determined using antibiotic discs diffusion method. Results: The results showed that Biofresh K is the best in the term of bacterial killing as its inhibition activity continued to 1:16 dilution and when the sensitivity of T.denticola isolates to some antibiotics was tested, it was appeared that ciprofloxacin is the best causing growth inhibition with the lowest minimum inhibitory cocentration (0.0001mg), and when the synergistic effect of the tested antibiotics was studied it is becoming clear that the lowest antibiotic concentrations can cause growth inhibition when the two antibiotics (mitronidazole + ciprofloxacin) or (mitronidazole + amoxillin) are used in combination. Conclusions: It is possible to depend on culturing methods for determining the sensitivity of the bacterium T.denticola to chemotherapeutic drugs. Biofresh K is the best among the rinses under study and the antibiotic CIP is the best one with the lowest MIC and the lowest concentration of the antibiotics resulting in growth inhibition can be achieved when they are used in combination