Preparation and pH with Antibacterial Evaluation of Nano Calcium Oxide Based Intracanal Medicament from Natural Products (An In vivo Study)

Abstract

Background To prepare new calcium based intracanal medicament from egg shell and sea shell powder mixed with pure coconut oil then evaluate the antibacterial activity of the new prepared materials and compare the result with Metapex intracanal medicament. Materials and Methods Calcium based intracanal medicament have been prepared from local egg shell and from seashell (Cowrie) in a form of nano calcium oxide powder; each powder was mixed separately with locally made pure coconut oil to form two experimental materials. Antimicrobial activity was evaluated by direct contact inhibition zone test of each experimental material and control with Enterococcus faecalis, after 24 hours of incubation at 37◦C, zone of bacterial growth was observed and measured. Results The results showed that both of the prepared materials have antibacterial effect, these results were statistically analyzed using One-way ANOVA & Duncan›s Multiple Range test at p=0.01 levels. Conclusion Results have shown that both egg and sea shells contain many types of metal oxides in different concentration that might have antibacterial action in addition to the lauric acid from the coconut oil which clinically proven to have antibacterial activity that aided the experimental materials to have an acceptable antibacterial activity.