Antimicrobial activity of Leuconostoc mesenteroides biofilm against different microorganisms


Back ground: Leuconostoc is one of the species of lactic acid bacteria that produced biofilms.Probiotic bacteria that produced biofilm has been used as naturopathy against different microbial pathogens.Objective: This study was conducted to determine the antimicrobial activity of Leuconostoc biofilm , against 24 isolates (4 of 6 different types) of food borne pathogens including Staphylococcus aureus , Salmonella spp, Escherichia coli , Klebsiella spp , Pseudomonas aeruginosa , Streptococcus mutans , Bacillus subtillus , Bacillus cereus, Bacillus sterothermophillus and Candida albicans.Materials and methods: using various concentration in vitro by filter paper disk diffusion method .Result: The present study showed the potent antimicrobial activity of the Leuconostoc mesenteroides biofilm against the all tested bacterial pathogens except Bacillus species and yeast Candida albicans. Biofilm produced by Leuconostoc mesenteroides showed highest zone of inhibition (13mm) against Escherichia coli and lowest zone of inhibition (7.0mm) against Streptococcus mutans .Conclusion: Consequently, Leuconostoc mesenteroides biofilm may be used as an antimicrobial agent in food products to prevent spoilage.