Detection of AGT Gene Polymorphism in Patient with Hypertension in Mosul City

Abstract

Polymorphisms in the promoter region of the angiotensinogen (AGT) gene may affect AGT transcription and level of blood pressure. We determined the frequency of the AGT polymorphism in sample of Iraqi patients with primary hypertension. Using a molecular epidemiology approach, we also determined the relationship between primary hypertension and environmental-AGT polymorphism interactions of this study was to investigate the association between genetic polymorphisms of AGT M235T genes and Hypertension in Mosul city. Venous blood samples were collected from each subject in two separate test tubes: one was used for biochemical analysis. The other was collected in EDTA tube for DNA extraction. Genomic DNA was isolated from whole-blood samples of all the patients and control subjects. DNA concentration and purity were determined measuring by Bio drop and detected the optimum DNA concentration for PCR analysis. The quality of the DNA was determined using agarose gel electrophoresis stained with ethidium bromide, samples were stored at -20 °C until further use. The current study showed increase biochemical parameters in female patients compare with male patients. Regarding AGT/M235T gene polymorphism, In the present study, AGT/MT genotyping revealed that 47.5 % of patients, AGT/MM homozygous had 42.6 % and AGT/TT homozygous had 9.8 % in patients with Hypertension.