Detection of Epstein - Barr virus Type1 and Type2 in Subtypes of Non- Hodgkin Lymphoma in Kerbela Province by Using Polymerase Chain Reaction


Epstein-Barr virus (EBV) is a ubiquitous virus associated with lymphomas. However, in Kerbala as other parts of Iraq, EBV detection is not a part of the workup for lymphoma diagnosis. . Archival formalin-fixed paraffin- embedded blocks from 44 patients with mean age 45.9±22.8 (range from 4 to 86years) diagnosed as Non Hodgkin lymphoma during the period from 2010 to 2018 in Kerbala city included in this study. Histological diagnosis was reviewed. Around 25mg of archival tissue were used to extract DNA. DNA extracts were used Polymerase Chain Reaction (PCR) targeting the EBNA3C region of the viral genome. This PCR system offered detection and typing of the EBV. T-test and chi-square were used to evaluate the association between age with the subtype of Non Hodgkin lymphoma, gender and EBV state. The majority of the Non Hodgkin lymphomas(NHL) belonged diffuse large B cell (DLBC) [n=28, 63.6%], T-cell rich Diffuse large B-cell [n=9, 20.5%], high grade B cell Burkitt- like (BL) [n=3, 6.8%], small Blymphocytic [n=2,4.5%],high grade B cell(CD20-ve,CD30-ve[n=1,2.3%] and peripheral T-cell [n=1,2.3%]. The other subtypes of NHL were not reported in this study. Male and female were equal affected with NHL (22 for each) and the peak age range of NHL over 60 years. EBV was detected in 21(47%) out of all cases. EBV type 1 was more common to EBV2in all subtype of NHL. To our knowledge, this is the first- time such data has been generated in Iraq. In this study present evidence that Non- Hodgkin lymphoma cases in Kerbela are associated with EBV1 and EBV2 types. There is a difference in the distribution of the EBV types according to the subtypes of lymphoma. The aim of this study: to detect types of EBV in the subtype of Non- Hodgkin lymphoma (NHL) among patients from Kerbala province, Iraq