تأثير الخمج بداء المقوسات في تركيز الانترفيرون كاما والبروتينات المتممة C3,C4 للنساء المخمجات بطفيلي Toxoplasma gondii

Abstract

Entamoeba histolytica is an enteric protozoan parasite which caused tissue invasive amoebiasis. It can induce the production of cytokines (such as IL-8 and TNF-α) and chemokines (such as MCP-1) from intestinal epithelial cells. This study was done to detect the serum level of IL-8, MCP-1 and TNF-α, using an enzyme-linked immunosorbent assay, in 60 children affected by amoebiasis to correlate the production of these cytokines with infection and comparison with the healthy persons (n= 30). Three aged groups (1 month- 2 year, 2-4 year and 4-6 year) were entered in the study for patients and healthy controls. The level of IL-8 (37.22 ± 7.06, 37.35 ± 1.981 and 36.45 ± 4.39 pg/ml) in children with amoebiasis were higher significantly compared with the level in healthy children (29.95 ± 4.30, 26.90 ± 4.17 and 25.32 ± 2.31 pg/ml) in all age groups (1 month- 2 year, 2-4 year and 4-6 year, respectively). Significant differences in the level of MCP-1 which were higher (28.92 ± 1.48, 30.85 ± 3.78 and 31.91 ± 6.82 pg/ml) in children with amoebiasis than in healthy children (18.44 ± 0.74, 22.62 ± 6.25 and 18.30 ± 1.43 pg/ml) in all age groups (respectively). Also, the level of TNF-α were higher significantly (58.05 ± 6.90, 55.87 ± 3.81 and 57.32 ± 8.86 pg/ml) in children with amoebiasis compared with healthy children (42.91 ± 2.54, 43.54 ± 3.42 and 42.64 ± 3.47 pg/ml, respectively) in all age groups (respectively). These findings reflect a role of IL-8, MCP-1 and TNF-α in E. histolytica infection.