EVALUATION OF THE ROLE OF CHITOSAN IN THE IMMUNOPATHOLOGY OF OSTEOMYELITIS IN DIABETIC RABBITS

Abstract

This study was conducted to investigate the beneficial effects of chitosan in theimmunopathology of osteomyelitis in diabetic rabbits; therefore, the experimentaldesign was carried out on 40 rabbits. They were divided into 5 groups each of 8animals, diabetes mellitus was induced in rabbits , then infected by Staphylococcusaureus and treated as following: First group (G1) was induced diabetes mellitus thenimmunized by whole sonicated S. aureus antigens (WSSAG) and inducedexperimentally osteomyelitis. The second group (G2) was induced diabetes mellitus,then immunized by (WSSAG) and induced experimentally osteomyelitis and fed ondiet containing chitosan. Third group (G3) was induced diabetes mellitus, and inducedexperimentally osteomyelitis only. Fourth group (G4) was induction of diabetesmellitus, and induced experimentally osteomyelitis and fed on diet containingchitosan. Fifth group (G5) was induced experimentally osteomyelitis only withoutdiabetes mellitus induction. Then at day 28th - 30th post immunization, skin test wasperformed to each of the immunized groups, and at day 30th the antibodies titer wasmeasured by passive hemagglutination assay and phagocytic activity, then the animalswere sacrificed and the treated bone taken for histopathological examination. In thepresent study , a significant increase was noted in the value of skin thickness of G2 at48 and 72 hrs PI.