Isolation, Screening and Identification of Low Density Polyethylene (LDPE) degrading bacteria from contaminated soil with plastic wastes

Abstract

One hundred and sixty nine bacterial isolates were isolated from 64 soil and plastic waste samples from different municipal landfill regions in Baghdad city. Primary screening has been done for the bacterial isolates depending on bacterial growth (OD600nm) in low density polyethylene solid and liquid medium. Forty two isolates show high efficient to degrade LDPE powder, then secondary screening has been done depending on bacterial growth, color change in solid medium, and change in pH medium. Bacterial isolates Mw43, Tw53 and Shw51were given high ability to degrade LDPE strips. These three isolates have been identified as Pseudomonas fluorescens، Pseudomonas aeruginosa and Acinetobacter ursingii, respectively.