Comparison between SSR and RFLP techniques for genetic diversity of Iraqi date palm (Phoenix dactylifera L.)


This study used 5 microsatellites to determinate genetic diversity among sixty five date palm cultivars, profile of all of these by separated Simple Sequence Repeat SSR-PCR products on the gel electrophoresis either one band (homozygous) or two bands (heterozygous) were revealed for each primer based on the types of microsatellite loci. And Restriction Fragment Length Polymorphism RFLP- PCR products separated on the gel acrylamide. The results in this study revealed that the total number of allele with 5 microsatellites was 95 alleles, average of amplified bands was 66 bands and the percentage of polymorphic bands was 77%. The results of matrix revealed the similarity between date palm cultivars with SSR primers represented by the highest distance was (1.2371) between (Smeasmi) and (Sabb Drrah), that means no similarity with them cultivars, while the lowest degree of distance was (zero), which represented no distance between cultivars were, (Shwethi) and (Maktom), that means presence of similarity between them. Genetic relationships among twenty four date palm cultivars estimated by SSR primers from unweighted pair-group method (UPGMA) dendrogram. Another wise this study used universal primers internal transcribed 2. spacer ITS Forward and ITS Reverse, The analysis was performed to reveal varieties fingerprinting with the Sultana, Sukkary and Maktom cultivars that mean number of fingerprinting was 3.The results of RFLP in this study revealed that the total number of amplified bands were 48 bands and the percentage of polymorphic bands were 100%.