A field experiment was conducted at farm of Field Crop Department College of Agriculture, University of Baghdad Al-jadiriyah during the summer season 2016. The aim was to study the effect of seeds soaking in Benzyl Adenine (BA), Indole butyric acid (IBA), Cobalt (Co) individually or combined in yield ,yield component and quality of two varieties of sesame. The experiment was applied according to the arrangement of Split-plots in randomized complete block design (R.C.B.D) with three replications. The main plots included two varieties (Wadaa,Sumer) while the Sub-plots were used for seed soaking treatment: Control (soaking in distilled water), BA solution (50 mg L-1), IBA solution (100 mg L-1), Co solution (2 mgL-1), and their combination. Where the treatment of soaking Individually with IBA significantly in increase in number of capsule per, oil and protein percentage in the seeds (122.40 capsule, %51.17, (%19.59respectively. Whereas Seed soaking with BA and Co separately recorded highest seed yield an increment in and percentage of had accrue it reached 68.55, 56.12% respectively compared with the (soaking in water).This superiority was a result of the superiority of the components of the yield, effect was also clear in decrease the percentage of charcoal rot infection at 21.77% and 24.14% respectively. The combination of (BA+IBA+Co) was significant in all the studied characters yield and seed yield, which was the highest among the other soaking treatment for the total seed yield reaches 1178.50 kg h-1 compared with control (socking in water). The Sumer variety was superior in seed yield, it reached 1215.96 kgh-1, however it gave highest percentage of oil reached 52.32% and was decreased charcoal rot percentage reaching 4.70%. The results showed that the best combination of interaction between seed soaking and two varieties was when planting Sumer with (BA+IBA) and (BA+IBA+Co) to give high seed yield reaching 1440.30 and 1324.70 kgh-1,the highest percentage of oil and protein with no infection with of charcoal rot pathogen.