Preparation of novel Azo Dyes as a new anti-Human Breast Cancer MDA-MB231 Cells and study its association with DNA

Abstract

The 4,6-bis((E)-(3-nitrophenyl)diazenyl) benzene-1,3-diol (1), 4-((E)-(3-nitrophenyl)diazenyl)-6-((E)-(4-nitrophenyl)diazenyl)benzene -1,3-diol (2), 4-((E)-(2-nitrophenyl) diazenyl)-6-((E)-(3-nitrophenyl)diazenyl)benzene-1,3-diol (3), 4,6-bis((E)-(2-nitrophenyl) diazenyl)benzene-1,3-diol (4), (N-(4-hydroxy-3-(((4-(5-(p-tolyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl)phenyl)sulfonyl)diazenyl)phenyl)acetamide (5), 3-(isopropylamino)-1-(4-(2-methoxyethyl)phenoxy)-1-((3-nitrophenyl)diazenyl)propan-2-ol (6), 3-(isopropylamino)-1-(4-(2-methoxyethyl)phenoxy)-1-((2-nitrophenyl)diazenyl)propan-2-ol (7) and 4-(((4-(5-(p-tolyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl)phenyl)sulfonyl) diazenyl)benzene-1,3-diol (8) were synthesised. The resulting diazo dyes and pharmaceutical azo dyes were then characterized using (m.p., IR, UV-visible and mass spectrum) and (m.p., IR, UV-visible and 1H NMR spectrum) respectively. Add to which, all the synthetic azo dyes were provided non-toxic effects using different concentrations from each, and didn't show any hemolysis effect in the cells. The cell viability (cytotoxicity assay) is used for each dye to observe their ability in destroying cancer living cells. And reduce its growth for human breast MDA-MB231 cancer cells, after 24h treatment with 100µM of each dye. The results were showed well activities of each dye against cell viability in contrast with the control. Further, the results of human DNA binding of each azo dye were indicated its ability to damage DNA and inhibiting of DNA transcription and replication.These results with that obtained by the NanoDrop™ spectrophotometer were showed increasing in the concentration of the nitrogen bases, which confirmed the DNA was damaged. Due to recommend the synthetic, non-toxic azo dyes as novel drugs for treatment of human breast MDA-MB231 cancer cells through its ability to destroy the DNA of the cancer cells.