Abstract

Two different plant extracts, thyme (Thymus vulgaris) and eucalyptus (Eucalyptus sp.), two bacterial strains, Pseudomonas fluorescens L18 and Bacillus subtilis K3, one antibiotic (Streptomycin) and one bactericide (copper sulphate) were tested for their control efficacy against Tomato Bacterial Spot caused by the bacterium Xanthomonas campestirs pv.vesicatoria under vitro and in vivo conditions. Two different concentrations of each extract was used, under lab and greenhouse conditions. The eucalyptus extract had better effect than the thyme extract in both conditions. The inhibition zone in Petri dishes was 11.25 mm and 6.99 mm and the reduction of disease severity index in plants was 40.7% and 35.6% respectively.The Pseudomonas fluorescens L18 strain had much better and was more effective than the Bacillus subtilis K3 strain in reduction the disease severity in plants. The disease severity index was reduced by 29.3% and 18.2% respectively. Streptomycin used as antibiotic had a great effect on reduction of the disease severity index 83.5% and inhibition zone 22.5mm. Copper sulphate reduced the disease severity index by 87% and had 38.1mm inhibition zone and had the best effect compared with other treatments.

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The article was added to IASJ on 2020-04-07

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